KMID : 0880220100480020257
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Journal of Microbiology 2010 Volume.48 No. 2 p.257 ~ p.261
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Isolation and Characterization of Biogenic Amine-Producing Bacteria in Fermented Soybean Pastes
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Moon Jin-Seok
Cho Seung-Kee Choi Hwa-Young Kim Ji-Eun Kim So-Young Cho Kyung-Ju Han Nam-Soo
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Abstract
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Biogenic amines (BAs) are produced primarily by microorganisms found in fermented foods and are often implicated in food poisoning. BA-producing bacteria found in fermented soybean pastes were isolated and characterized using a decarboxylating medium and multiplex PCR analysis. Two BA-producing bacteria were isolated from traditional soybean pastes: one was a histamine-producing Clostridium strain, and the other was a tyramine-producing Pseudomonas strain. The Clostridium strain was determined to be a potent histamine producer among the cultures tested. Synthesis of tyramine by Pseudomonas sp. T1 was observed for the first time in this study.
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KEYWORD
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biogenic amines, histamine, tyramine, multiplex PCR, Clostridium sp., Pseudomonas sp.
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